首頁 > 儀器試劑 > Reagent 試劑 > Agilent > 細胞生物相關 > PathDetect Cis-Reporting Systems

PathDetect Cis-Reporting Systems

細胞訊息傳遞報導系統


細胞訊息傳遞檢測系統 (Cis)

透過安捷倫 PathDetect Cis-Reporting 系統,您可以解析基因產物或是分子物質是否會影響到特定的下游 enhancer。只需要將 PathDetect cis-reporting 載體與您實驗所感興趣的基因表現載體共同轉染至動物細胞中,經由報導基因的表現差異,就能了解您的基因是否能驅動下游的 enhancer element 或訊息表現。

PathDetect Cis-Reporting Systems 如何運作細胞外來的訊號刺激可驅動一連串的細胞內訊息分子傳導,例如 protein kinases 或 phophatases,許多轉錄因子 (transcription factors) 經由這些訊息傳遞影響會結合至特定的 enhancer element,進而影響之後的基因表現。相同的實驗理論,用戶可將感興趣的分子或基因送入細胞,並透過系統的 luciferase reporter 基因表現強度來偵測此外來物是否能影響此特定 enhancer element。

另有終極彈性的 pLuc-MCS 表現載體,裡面包含 multiple cloning site (MCS) 可依照您的實驗需求插入別的 enhancer 或 transcription element。系統中的 positive control 及 negative control 載體都可額外添購,加速您的實驗效率。

載體系統資訊與實驗效能

透過 Luciferase 表現來偵測轉錄活性1 µg 的 pNF-kb-Luc plasmid 和各種不同濃度的 pFC-MEKK plasmid (positive control) 共同轉染至 HeLa cells 中,luciferase 的活性於轉染後的 48 個小時開始測定,從實驗圖可看出,轉染pFC-MEKK 的細胞會有倍數的 luciferase 表現。

提供 Luciferase 及 hrGFP 兩種 reporting 系統

表現載體

cis-Report plasmids (Luciferase reporter)

cis-Report plasmids (hrGFP reporter)

pLuc-MCS (Luciferase reporter)

PathDetect Cis-Reporting System 總覽

AP-1Activator protein-1
c/EBPCCAAT-enhancer binding protein
CRECyclic AMP
DR1/RXRERetinoic x receptor epsilon
DR3/VDRVitamin D receptor
DR4/TRThyroid receptor
DR5/RARERetinoic acid receptor epsilon
Egr-1Early growth response-1
GASGamma-activated sequence
GREGlucocorticoid response element
ISREInterferon-stimulated response element
LILRELPS IL-1beta response element
NFATNuclear factor of activated T cells
NF-κBNuclear factor κB
p53Tumor suppressor protein p53
SRESerum response element
SRFSerum response factor
TARETGF-Beta or Activin response element

產品列表

貨號 產品 數量
219090 PathDetect pCIS-CK Negative Control Plasmid PathDetect pCIS-CK 陰性對照質體(組) 廠牌:Agilent 50 μg
219071 PathDetect pFC-PKA Positive Control Plasmid PathDetect pFC-PKA 陽性對照質體(組) 廠牌:Agilent 10 μg
219059 PathDetect pFC-MEKK Positive Control Plasmid PathDetect pFC-MEKK 陽性對照質體(組) 廠牌:Agilent 10 μg
219084 PathDetect pFC-p53 Positive Control Plasmid PathDetect pFC-p53 陽性對照質體(組) 廠牌:Agilent 10 μg
219087 PathDetect pLuc-MCS plasmid cis-Reporter Plasmid PathDetect pLuc-MCS 質體 cis-報導者質體(組) 廠牌:Agilent 50 μg
240053 PathDetect pNFAT-hrGFP Cis-Reporting Plasmid PathDetect pNFAT-hrGFP 質體 cis-報導者質體(組) 廠牌:Agilent 50 μg
240051 PathDetect pNF-kB-hrGFP Cis-Reporting Plasmid PathDetect pNF-kB-hrGFP 質體 cis-報導者質體(組) 廠牌:Agilent 50 μg
240050 PathDetect pCRE-hrGFP Cis-Reporting Plasmid PathDetect pCRE-hrGFP cis-報導者質體(組) 廠牌:Agilent 50 μg
240049 PathDetect pAP-1-hrGFP Cis-Reporting Plasmid PathDetect pAP-1-hrGFP cis-報導者質體(組) 廠牌:Agilent 50 μg
240136 PathDetect DR4/TR Cis-Reporting Plasmid PathDetect DR4/TR cis-報導者質體(組) 廠牌:Agilent 50 μg
240134 PathDetect GRE Cis-Reporting Plasmid PathDetect GRE cis-報導者質體(組) 廠牌:Agilent 50 μg
240132 PathDetect LILRE Cis-Reporting Plasmid PathDetect LILRE cis-報導者質體(組) 廠牌:Agilent 50 μg
240130 PathDetect Egr-1 Cis-Reporting Plasmid PathDetect Egr-1 cis-報導者質體(組) 廠牌:Agilent 50 μg
240120 PathDetect DR5/RARE Cis-Reporting Plasmid PathDetect DR5/RARE cis-報導者質體(組) 廠牌:Agilent 50 μg
240116 PathDetect DR3/VDR Cis-Reporting Plasmid PathDetect DR3/VDR cis-報導者質體(組) 廠牌:Agilent 50 μg
240114 PathDetect DR1/RXRE Cis-Reporting Plasmd PathDetect DR1/RXRE cis-報導者質體(組) 廠牌:Agilent 50 μg
240112 PathDetect c/EBP Cis-Reporting Plasmid PathDetect c/EBP cis-報導者質體(組) 廠牌:Agilent 50 μg
240039 PathDetect pTARE-Luc Plasmid PathDetect pTARE-Luc質體(組) 廠牌:Agilent 50 μg
219088 PathDetect NFAT Cis-Reporter Plasmid PathDetect NFAT cis-報導者質體(組) 廠牌:Agilent 50 μg
219091 PathDetect pGAS-Luc Plasmid PathDetect pGAS-Luc質體(組) 廠牌:Agilent 50 μg
219089 PathDetect pISRE-Luc Plasmid PathDetect pISRE-Luc質體(組) 廠牌:Agilent 50 μg
219085 PathDetect p53-Luc Plasmid PathDetect p53-Luc質體(組) 廠牌:Agilent 50 μg
219082 PathDetect pSRF-Luc Plasmid PathDetect pSRF-Luc質體(組) 廠牌:Agilent 50 μg
219080 PathDetect pSRE-Luc Plasmid PathDetect pSRE-Luc質體(組) 廠牌:Agilent 50 μg
219078 PathDetect pNFkB-Luc Plasmid PathDetect pNFkB-Luc質體(組) 廠牌:Agilent 50 μg
219076 PathDetect pCRE-Luc Plasmid PathDetect pCRE-Luc質體(組) 廠牌:Agilent 50 μg
219074 PathDetect pAP1-Luc Plasmid PathDetect pAP1-Luc質體(組) 廠牌:Agilent 50 μg
240135 PathDetect DR4 Cis-Reporting System PathDetect DR4 Cis-報導者系統(組) 廠牌:Agilent 50 assays
240133 PathDetect GRE Cis-Reporting System PathDetect GRE Cis-報導者系統(組) 廠牌:Agilent 50 assays
240131 PathDetect LILRE Cis-Reporting System PathDetect LILRE Cis-報導者系統(組) 廠牌:Agilent 50 assays
240129 PathDetect Egr-1 Cis-Reporting System PathDetect Egr-1 Cis-報導者系統(組) 廠牌:Agilent 50 assays
240119 PathDetect DR5/RARE Cis-Reporting System PathDetect DR5/RARE Cis-報導者系統(組) 廠牌:Agilent 50 assays
240115 PathDetect DR3/VDR Cis-Reporting System PathDetect DR3/VDR Cis-報導者系統(組) 廠牌:Agilent 50 assays
240113 PathDetect DR1/RXRE Cis-Reporting System PathDetect DR1/RXRE Cis-報導者系統(組) 廠牌:Agilent 50 assays
240111 PathDetect c/EBP Cis-Reporting System PathDetect c/EBP Cis-報導者系統(組) 廠牌:Agilent 50 assays
219094 PathDetect NFAT Cis-Reporting System PathDetect NFAT Cis-報導者系統(組) 廠牌:Agilent 50 assays
219093 PathDetect GAS Cis-Reporting System PathDetect GAS Cis-報導者系統(組) 廠牌:Agilent 50 assays
219092 PathDetect ISRE Cis-Reporting System PathDetect ISRE Cis-報導者系統(組) 廠牌:Agilent 50 assays
219083 PathDetect p53 Cis-Reporting System PathDetect p53 Cis-報導者系統(組) 廠牌:Agilent 50 assays
219081 PathDetect SRF Cis-Reporting System PathDetect SRF Cis-報導者系統(組) 廠牌:Agilent 50 assays
219079 PathDetect SRE Cis-Reporting System PathDetect SRE Cis-報導者系統(組) 廠牌:Agilent 50 assays
219077 PathDetect NF-kB Cis-Reporting System PathDetect NF-kB Cis-報導者系統(組) 廠牌:Agilent 50 assays
219075 PathDetect CRE Cis-Reporting System PathDetect CRE Cis-報導者系統(組) 廠牌:Agilent 50 assays
219073 PathDetect AP-1 Cis-Reporting System PathDetect AP-1 Cis-報導者系統(組) 廠牌:Agilent 50 assays

PathDetect Cis-Reporting Systems

  • 可檢測特定訊息轉錄活化的路徑
  • 簡單快速,比 blotting 及 gel-shift assay 更為方便
  • 高敏感性,可定量的實驗系統

說明書下載

219073/ 219075/ 219077/ 219079/ 219081/ 219083/ 219092/ 219093/ 219094/ 219095/ 240111/ 240113/ 240115/ 240119/ 240129/ 240131/ 240133/ 240135/ 240049/ 240050/ 240051/ 240053/ 219087 PathDetect cis-reporting systems使用手冊
回到頂端