PathDetect Cis-Reporting Systems
細胞訊息傳遞報導系統
細胞訊息傳遞檢測系統 (Cis)
透過安捷倫 PathDetect Cis-Reporting 系統，您可以解析基因產物或是分子物質是否會影響到特定的下游 enhancer。只需要將 PathDetect cis-reporting 載體與您實驗所感興趣的基因表現載體共同轉染至動物細胞中，經由報導基因的表現差異，就能了解您的基因是否能驅動下游的 enhancer element 或訊息表現。
PathDetect Cis-Reporting Systems 如何運作細胞外來的訊號刺激可驅動一連串的細胞內訊息分子傳導，例如 protein kinases 或 phophatases，許多轉錄因子 (transcription factors) 經由這些訊息傳遞影響會結合至特定的 enhancer element，進而影響之後的基因表現。相同的實驗理論，用戶可將感興趣的分子或基因送入細胞，並透過系統的 luciferase reporter 基因表現強度來偵測此外來物是否能影響此特定 enhancer element。
另有終極彈性的 pLuc-MCS 表現載體，裡面包含 multiple cloning site (MCS) 可依照您的實驗需求插入別的 enhancer 或 transcription element。系統中的 positive control 及 negative control 載體都可額外添購，加速您的實驗效率。
載體系統資訊與實驗效能
透過 Luciferase 表現來偵測轉錄活性1 µg 的 pNF-kb-Luc plasmid 和各種不同濃度的 pFC-MEKK plasmid (positive control) 共同轉染至 HeLa cells 中，luciferase 的活性於轉染後的 48 個小時開始測定，從實驗圖可看出，轉染pFC-MEKK 的細胞會有倍數的 luciferase 表現。
提供 Luciferase 及 hrGFP 兩種 reporting 系統
cis-Report plasmids (Luciferase reporter)
cis-Report plasmids (hrGFP reporter)
pLuc-MCS (Luciferase reporter)
PathDetect Cis-Reporting System 總覽
| AP-1 | Activator protein-1 |
| c/EBP | CCAAT-enhancer binding protein |
| CRE | Cyclic AMP |
| DR1/RXRE | Retinoic x receptor epsilon |
| DR3/VDR | Vitamin D receptor |
| DR4/TR | Thyroid receptor |
| DR5/RARE | Retinoic acid receptor epsilon |
| Egr-1 | Early growth response-1 |
| GAS | Gamma-activated sequence |
| GRE | Glucocorticoid response element |
| ISRE | Interferon-stimulated response element |
| LILRE | LPS IL-1beta response element |
| NFAT | Nuclear factor of activated T cells |
| NF-κB | Nuclear factor κB |
| p53 | Tumor suppressor protein p53 |
| SRE | Serum response element |
| SRF | Serum response factor |
| TARE | TGF-Beta or Activin response element |
產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| 219090 | PathDetect pCIS-CK Negative Control Plasmid PathDetect pCIS-CK 陰性對照質體(組) 廠牌:Agilent | 50 μg | |
| 219071 | PathDetect pFC-PKA Positive Control Plasmid PathDetect pFC-PKA 陽性對照質體(組) 廠牌:Agilent | 10 μg | |
| 219059 | PathDetect pFC-MEKK Positive Control Plasmid PathDetect pFC-MEKK 陽性對照質體(組) 廠牌:Agilent | 10 μg | |
| 219084 | PathDetect pFC-p53 Positive Control Plasmid PathDetect pFC-p53 陽性對照質體(組) 廠牌:Agilent | 10 μg | |
| 219087 | PathDetect pLuc-MCS plasmid cis-Reporter Plasmid PathDetect pLuc-MCS 質體 cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240053 | PathDetect pNFAT-hrGFP Cis-Reporting Plasmid PathDetect pNFAT-hrGFP 質體 cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240051 | PathDetect pNF-kB-hrGFP Cis-Reporting Plasmid PathDetect pNF-kB-hrGFP 質體 cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240050 | PathDetect pCRE-hrGFP Cis-Reporting Plasmid PathDetect pCRE-hrGFP cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240049 | PathDetect pAP-1-hrGFP Cis-Reporting Plasmid PathDetect pAP-1-hrGFP cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240136 | PathDetect DR4/TR Cis-Reporting Plasmid PathDetect DR4/TR cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240134 | PathDetect GRE Cis-Reporting Plasmid PathDetect GRE cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240132 | PathDetect LILRE Cis-Reporting Plasmid PathDetect LILRE cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240130 | PathDetect Egr-1 Cis-Reporting Plasmid PathDetect Egr-1 cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240120 | PathDetect DR5/RARE Cis-Reporting Plasmid PathDetect DR5/RARE cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240116 | PathDetect DR3/VDR Cis-Reporting Plasmid PathDetect DR3/VDR cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240114 | PathDetect DR1/RXRE Cis-Reporting Plasmd PathDetect DR1/RXRE cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240112 | PathDetect c/EBP Cis-Reporting Plasmid PathDetect c/EBP cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 240039 | PathDetect pTARE-Luc Plasmid PathDetect pTARE-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 219088 | PathDetect NFAT Cis-Reporter Plasmid PathDetect NFAT cis-報導者質體(組) 廠牌:Agilent | 50 μg | |
| 219091 | PathDetect pGAS-Luc Plasmid PathDetect pGAS-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 219089 | PathDetect pISRE-Luc Plasmid PathDetect pISRE-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 219085 | PathDetect p53-Luc Plasmid PathDetect p53-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 219082 | PathDetect pSRF-Luc Plasmid PathDetect pSRF-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 219080 | PathDetect pSRE-Luc Plasmid PathDetect pSRE-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 219078 | PathDetect pNFkB-Luc Plasmid PathDetect pNFkB-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 219076 | PathDetect pCRE-Luc Plasmid PathDetect pCRE-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 219074 | PathDetect pAP1-Luc Plasmid PathDetect pAP1-Luc質體(組) 廠牌:Agilent | 50 μg | |
| 240135 | PathDetect DR4 Cis-Reporting System PathDetect DR4 Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 240133 | PathDetect GRE Cis-Reporting System PathDetect GRE Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 240131 | PathDetect LILRE Cis-Reporting System PathDetect LILRE Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 240129 | PathDetect Egr-1 Cis-Reporting System PathDetect Egr-1 Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 240119 | PathDetect DR5/RARE Cis-Reporting System PathDetect DR5/RARE Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 240115 | PathDetect DR3/VDR Cis-Reporting System PathDetect DR3/VDR Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 240113 | PathDetect DR1/RXRE Cis-Reporting System PathDetect DR1/RXRE Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 240111 | PathDetect c/EBP Cis-Reporting System PathDetect c/EBP Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219094 | PathDetect NFAT Cis-Reporting System PathDetect NFAT Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219093 | PathDetect GAS Cis-Reporting System PathDetect GAS Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219092 | PathDetect ISRE Cis-Reporting System PathDetect ISRE Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219083 | PathDetect p53 Cis-Reporting System PathDetect p53 Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219081 | PathDetect SRF Cis-Reporting System PathDetect SRF Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219079 | PathDetect SRE Cis-Reporting System PathDetect SRE Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219077 | PathDetect NF-kB Cis-Reporting System PathDetect NF-kB Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219075 | PathDetect CRE Cis-Reporting System PathDetect CRE Cis-報導者系統(組) 廠牌:Agilent | 50 assays | |
| 219073 | PathDetect AP-1 Cis-Reporting System PathDetect AP-1 Cis-報導者系統(組) 廠牌:Agilent | 50 assays |
PathDetect Cis-Reporting Systems
- 可檢測特定訊息轉錄活化的路徑
- 簡單快速，比 blotting 及 gel-shift assay 更為方便
- 高敏感性，可定量的實驗系統
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