PathDetect Trans-Reporting Systems 可以用來檢測特定的 pathway 中, 是否有新的基因、生長因子、測試藥物等的直接或間接影響,透過簡單的實驗方式即可從安捷倫提供的六種 transcription factor 來判斷您的蛋白或化合物是否產生作用,此系統提供 luciferase reporter vector,能利用共同轉染的方式來測定您感興趣的基因。
極致的實驗彈性,安捷倫同時提供擁有 MCS (multiple cloning site) 的 pFA-CMV 載體,您可以將任何感興趣的 transcription factor 接入此載體來進行反應。此外也提供 positive 及 negative control 來確保您的實驗順利。
透過 Luciferase 活性來測定訊息傳導pFR-Luc reporter plasmid (1 µg) 、 10 ng 的 trans-activator plasmid 和 50 ng 的 indicated control vector 用 lipfection 的方式來共同轉染至細胞中來測定 luciferase 的表現,pFC-dbd 載體為 negative control 只表現 GAL4 DNA binding domain。
Pathway-specific trans-activator plasmid 的結構
pFA trans-Activator Plasmids
pFR trans-Reporter Plasmids
pFA-CMV Plasmid
PathDetect Trans-Reporting System 總覽
ATF-2 | MAPK (mitogen-activated protein kinase) pathway |
c-Fos | JNK (c-Jun N-terminal kinase) pathway |
c-Jun | JNK (c-Jun N-terminal kinase) pathway |
CHOP/GADD153 | p38 kinase pathway |
CREB | PKA (cAMP-dependent kinase) pathway |
Elk1 | MAPK (mitogen-activated protein kinase) pathway |
貨號 | 產品 | 數量 | |
---|---|---|---|
219071 | PathDetect pFC-PKA Positive Control Plasmid PathDetect pFC-PKA 陽性對照質體(組) 廠牌:Agilent | 10 μg | |
219059 | PathDetect pFC-MEKK Positive Control Plasmid PathDetect pFC-MEKK 陽性對照質體(組) 廠牌:Agilent | 10 μg | |
219086 | Path Detect pFC-MEK3 Positive Control Plasmid PathDetect pFC-MEK3 陽性對照質體(組) 廠牌:Agilent | 10 μg | |
219056 | Path Detect pFC2-dbd Negative Control Plasmid PathDetect pFC2-dbd 陰性對照質體(組) 廠牌:Agilent | 10 μg | |
219065 | Path Detect pFC-MEK1 Positive Control Plasmid PathDetect pFC-MEK1 陽性對照質體(組) 廠牌:Agilent | 10 μg | |
219031 | PathDetect pFA-cFos trans-Reporter Activator Plasmid PathDetect pFA-cFos trans-報導者活化質體(組) 廠牌:Agilent | 10 μg | |
219026 | PathDetect pFA-ATF2 trans-Reporter Activator Plasmid PathDetect pFA-ATF2 trans-報導者活化質體(組) 廠牌:Agilent | 10 μg | |
219054 | PathDetect pFA-CHOP trans-Reporter Activator Plasmid PathDetect pFA-CHOP trans-報導者活化質體(組) 廠牌:Agilent | 10 μg | |
219068 | PathDetect pFA2-CREB trans-Reporter Activator Plasmid PathDetect pFA2-CREB trans-報導者活化質體(組) 廠牌:Agilent | 10 μg | |
219062 | PathDetect pFA2-Elk1 trans-Reporter Activator Plasmid PathDetect pFA2-Elk1 trans-報導者活化質體(組) 廠牌:Agilent | 10 μg | |
219053 | PathDetect pFA2-cJun trans-Reporter Activator Plasmid PathDetect pFA2-cJun trans-報導者活化質體(組) 廠牌:Agilent | 10 μg | |
219050 | PathDetect pFR-Luc trans-Reporter Plasmid PathDetect pFR-Luc trans-報導者活化質體(組) 廠牌:Agilent | 50 μg | |
219004 | PathDetect pFR-SEAP trans-Reporter Plasmid PathDetect pFR-SEAP trans-報導者活化質體(組) 廠牌:Agilent | 50 μg | |
219002 | PathDetect pFR-βGal trans-Reporter Plasmid PathDetect pFR-βGal trans-報導者活化質體(組) 廠牌:Agilent | 50 μg | |
219001 | PathDetect pFR-CAT trans-Reporter Plasmid PathDetect pFR-CAT trans-報導者活化質體(組) 廠牌:Agilent | 50 μg | |
219036 | PathDetect pFA-CMV Plasmid PathDetect pFA-CMV質體(組) 廠牌:Agilent | 20 μg | |
219015 | PathDetect CHOP trans-Reporting System PathDetect CHOP trans-報導者系統(組) 廠牌:Agilent | 50 assays | |
219010 | PathDetect CREB trans-Reporting System PathDetect CREB trans-報導者系統(組) 廠牌:Agilent | 50 assays | |
219005 | PathDetect Elk1 trans-Reporting System PathDetect Elk1 trans-報導者系統(組) 廠牌:Agilent | 20 μg | |
219000 | PathDetect c-Jun trans-Reporting System PathDetect c-Jun trans-報導者系統(組) 廠牌:Agilent | 50 assays |