AdEasy Viral Titer Kit

特色

The AdEasy Viral Titer Kit is a simple enzyme-linked immunoassay for the determination of adenoviral titers. The immunoassay detects an adenoviral capsid protein called hexon, which is required for adenoviral replication. Expression of the adenoviral hexon protein is dependent upon the E1 gene product. AD-293 and HEK2931 cells are human embryonic kidney cells transformed by sheared adenovirus type 5 DNA. These cell lines produce the adenovirus E1 gene in trans, allowing the expression of hexon, and thus the production of infectious virus particles when cells are transfected with E1-deleted adenovirus vectors.

The AdEasy viral titer kit can determine the titer of your stocks in 24 to 48 hours, compared to two weeks for traditional endpoint dilution assays. Titer determination is achieved in three simple steps. First, AD-293 or HEK293 cells are fixed 24 to 48 hours post-adenoviral infection, and then expression of the hexon protein is detected with an anti-hexon antibody. Next, a horseradish peroxidase (HRP) conjugated secondary antibody is added to amplify the signal. The HRP enzyme catalyzes a colorimetric reaction with the third reagent, a metal-enhanced substrate diaminobenzidine (DAB), which produces a dark precipitate in the cells expressing the hexon protein. Since only infected cells express the hexon protein, these are the only cells in culture that contain the dark brown precipitate indicative of the HRP-catalyzed reaction. The dark-colored, infected cells can therefore be counted under a standard laboratory microscope and used to calculate viral titer.

產品列表

AdEasy Adenoviral Vector System

• Determine the titer of your adenoviral stocks in 24-48 hours
• Simple enzyme-linked immunoassay

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