AdEasy Virus Purification Kit
AdEasy 病毒純化試劑套組
The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution. Concentrated and purified viral particles can be obtained in 2–3 hours, in contrast to traditional CsCl gradient centrifugation which typically takes 12–48 hours.
特色
The AdEasy Virus Purification Kit allows the purification and concentration of Adenovirus (from Ad5 strains) with Sartobind® syringe filters containing an ion exchange membrane adsorber that selectively binds adenoviral particles. Once bound, viral particles can be further purified by washing away nonspecifically-bound proteins before elution. Concentrated and purified viral particles can be obtained in 2–3 hours, in contrast to traditional CsCl gradient centrifugation which typically takes 12–48 hours (Figure 1). Ready-to-use 0.45 µm filters, Sartobind filter units, centrifuge concentrators, and buffers make the AdEasy virus purification procedure easy and efficient. For a typical adenoviral vector, 60 mL of cell culture (3x15 cm2 plates) purified using this method should yield approximately 1 – 3x1012 viral particles with an endotoxin level of < 0.025 EU/mL.
Figure 1: Easy to follow rapid protocol
產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| 240243 | AdEasy Virus Purification Kit AdEasy 病毒純化套組(組) 廠牌:Agilent | 2 x 100 mL |
AdEasy Virus Purification Kit
- Rapid alternative to cesium chloride (CsCl) density gradient methods
- No cesium chloride or ultracentrifugation necessary
- Increased infection rates and cell viability
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