pCMV-3Tag Vectors
分子標定蛋白表現載體
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. The pCMV-Tag vectors have a single copy of the available epitope tags, while the pCMV-3Tag vectors have three copies of the epitope tag (in a variety of configurations) to prove a stronger signal in immmunological assays. The small size of these tags ensures that they will have minimal, if any, interference with functional analysis studies.
特點介紹
Epitope-tagging technology provides you a powerful means to functionally analyze your protein of interest without the need for an antibody specific to each new protein under study. Our pCMV-3Tag vector series is the advanced epitope tagging system in which three copies of either the FLAG or c-myc tags are added to your protein. FLAG and c-myc tags are small, highly immunoreactive, do not interfere with the function of your protein, and are easily detected with commercially available antibodies. Three copies of each tag consistently enhance signal strength in all of your protein characterization studies: western blotting, in situ staining, and immunoprecipitation experiments.
Maximize Protein Expression Levels
In addition to the epitope tag sequences, the pCMV-3Tag vectors contain features for optimal expression of fusion proteins in mammalian cells. The cytomegalovirus immediate early (CMV) promoter allows constitutive expression of the cloned DNA in a wide variety of mammalian cell lines. A Kozak consensus sequence provides optimal expression of the fusion protein when the N-terminal vectors are used. Optimized protein expression levels combined with flexibility in cloning and tagging options, make this vector system the method of choice for your protein tagging and characterization studies (Figure 1).
Figure 1. We compared activity levels of the luciferase protein fused to one and three epitope tags.Versatility
Vectors conferring neomycin/kanamycin resistance to your transfected cells come in sets of three reading frames for each of the following tagging options: N-terminal FLAG, C-terminal FLAG, N-terminal c-myc, and C-terminal c-myc. Three reading frames allow the gene of interest to be fused correctly to the epitope tags. A hygromycin vector set for selection of stable transfectants is also available with each of the above tagging orientations.
產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| 240204 | pCMV-3Tag-9 Vector pCMV-3Tag-9載體(組) 廠牌:Agilent | 20 µg | |
| 240203 | pCMV-3Tag-8 Vector pCMV-3Tag-8載體(組) 廠牌:Agilent | 20 µg | |
| 240202 | pCMV-3Tag-7 Vector pCMV-3Tag-7載體(組) 廠牌:Agilent | 20 µg | |
| 240200 | pCMV-3Tag-6 Vectors pCMV-3Tag-6載體(組) 廠牌:Agilent | 20 µg | |
| 240198 | pCMV-3Tag-4 Epitope Tagging Mammalian Expression Vector pCMV-3Tag-4 抗原決定位標記哺乳動物表現載體(組) 廠牌:Agilent | 3 x 20 µg | |
| 240196 | pCMV-3Tag-2 Epitope Tagging Mammalian Expression Vector pCMV-3Tag-2 抗原決定位標記哺乳動物表現載體(組) 廠牌:Agilent | 3 x 20 µg | |
| 240195 | pCMV-3Tag-1 Epitope Tagging Mammalian Expression Vector pCMV-3Tag-1 抗原決定位標記哺乳動物表現載體(組) 廠牌:Agilent | 3 x 20 µg | |
| 240197 | pCMV-3Tag-3 Epitope Tagging Mammalian Expression Vector pCMV-3Tag-3 抗原決定位標記哺乳動物表現載體(組) 廠牌:Agilent | 3 x 20 µg | |
| 211170 | pCMV-Tag1 Vector pCMV-Tag1 載體(組) 廠牌:Agilent | 20 µg | |
| 211172 | pCMV-Tag2 Vector pCMV-Tag2 載體(組) 廠牌:Agilent | 3 x 20 µg | |
| 211173 | pCMV-Tag3 Vector pCMV-Tag3 載體(組) 廠牌:Agilent | 3 x 20 µg | |
| 211174 | pCMV-Tag4 Vector pCMV-Tag4 載體(組) 廠牌:Agilent | 3 x 20 µg | |
| 211175 | pCMV-Tag5 Vector pCMV-Tag5 載體(組) 廠牌:Agilent | 3 x 20 µg |
pCMV-3Tag Vectors
- Stronger signal in immunological assays
- Small tags do not interfere with functional analysis studies
- Three reading frames for both N- and C-terminus tags
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