Brilliant Multiplex QPCR Master Mix
多螢光即時定量基因分型試劑套組
The Brilliant Multiplex QPCR Master Mix allows you to amplify up to four targets in a single real-time PCR Reaction The Brilliant Multiplex QPCR Master Mix provides sufficient reaction components to accurately quantify both low-and-high-abundance targets in the same tube. This allows you to more successfully multiplex without concern for bias due to the abundance level. Importantly, the sensitivity remains equivalent to that seen in singleplex.
Get the Most Out of Rare or Limited Samples
Rare or limited samples, such as those obtained from biopsies or laser microdissection can be especially challenging to analyze. The Brilliant multiplex QPCR master mix provides amplification of up to four targets per reaction, and each requires far less template than if performed in four separate reactions. Moreover, the sensitivity remains equivalent to that seen in singleplex (Figure 1). This holds true across multiple dilutions of a standard curve, even at very low concentrations (Table 1). Thus, you maximize use of each precious sample without sacrificing sensitivity. In addition, the Brilliant multiplex master mix provides sufficient reaction components to accurately quantify both low and high abundance targets in the same tube.
Equivalent Performance with 4-Target MultiplexWe ran four targets—eNOS (FAM), HFE (HEX), CFTR (ROX), and Cyclophilin (Cy5)—in singleplex and multiplex using the Brilliant multiplex QPCR master mix on the Mx3000P real-time PCR system. Ct values for each target were virtually identical in the two reactions, indicating full sensitivity and performance in multiplex.
產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| 600553 | Brilliant Multiplex QPCR Master Mix | 200 rxn |
Brilliant Multiplex QPCR Master Mix
- Maximizes analysis of limited or rare samples
- Allows detection of multiple targets plus an internal control
- More economical per sample than singleplex, while saving time and increasing throughput
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