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EpiQuik Circulating Modified Histone H3 Multiplex Assay Kit(Colorimetric)

EpiQuik 循環修飾組蛋白 H3多重檢測套組


EpiQuik Circulating Modified Histone H3 Multiplex Assay Kit (Colorimetric) 為一完整的最佳化檢測試劑套組,可直接從血漿或血清中檢測 circulating Histone 修飾情形,ELISA-like 的 96 盤式設計能一次偵測且定量 22 種不同的Histone H3 組蛋白修飾,實驗步驟簡單方便。

背景介紹

Histone modifications have been defined as epigenetic modifiers. Post-translational modifications of histones include the acetylation of specific lysine residues by histone acetyltransferases (HATs), deacetylation by histone deacetylases (HDACs), methylation of lysine and arginine residues by histone methytransferases (HMTs), the demethylation of lysine residues by histone demethylases (HDMTs), and the phosphorylation of specific serine groups by histone kinases (HKs). Additional histone modifications include the attachment of ubiquitin (Ub), small ubiquitin-like modifiers (SUMOs), and poly ADP-ribose (PAR) units. Next to DNA methylation, histone acetylation and histone methylation are the most well characterized epigenetic marks. Generally, tri-methylation at H3-K4, H3-K36, or H3-K79 results in an open chromatin configuration and is therefore characteristic of euchromatin. Euchromatin is also characterized by a high level of histone acetylation, which is mediated by histone acetyltransferases. Conversely, histone deacetylases have the ability to remove this epigenetic mark, which leads to transcriptional repression. Condensed heterochromatin is enriched in tri-methylation of H3-K9 and H3-K27 and silencing of euchromatin loci caused by histone deacetylation involves the recruitment of specific K9 histone methyltransferases. Methylated H3-K9 provides a binding site for the chromodomain-containing heterochromatin protein 1 (HP1), which induces transcriptional repression and heterochromatinization. At euchromatic loci, this process is mediated by co-repressors, such as retinoblastoma protein pRb or KAP1. Histone demethylases have the opposite effect on transcription. For example, the histone demethylase LSD1 is responsible for H3K4 demethylation, which leads to transcriptional inactivation. Other histone demethylases, such as jumonji (JHDM2A), are responsible for H3K9 demethylation, whereas JHDM1 has the ability to convert active chromatin marks such as H3-K36me2, to an unmodified state. Lysine residues can be mono-, di-, or trimethylated, each of which can differentially regulate chromatin structure and transcription. Along with other histone modifications such as phosphorylation, this enormous variation leads to a multiplicity of possible combinations of different modifications. This may constitute a “histone code”, which can be read and interpreted by different cellular factors.

原理

This kit is designed for measuring multiple histone H3 modifications simultaneously from plasma and serum. In an assay with this kit, each histone H3 modified at specific sites will be captured by an antibody that is coated on the strip wells and specifically targets the appropriate histone modification pattern. The captured histone modified at specific sites will be detected with a detection antibody, followed by a color development reagent. The ratio of modified histone is proportional to the intensity of absorbance measured by a microplate reader at a wavelength of 450 nm.

適用樣品

Input Amount : The amount of plasma or serum for each assay can be 10 to 50 µl with an optimal amount of 30 µl.

實驗方式

EpiQuik Circulating Modified Histone H3 Multiplex Assay Kit實驗流程圖示

EpiQuik Circulating Modified Histone H3 Multiplex Assay Kit實驗原理示意圖

產品列表

貨號 產品 數量
P-3106-96 EpiQuik Circulating Modified Histone H3 Multiplex Assay Kit(Colorimetric) 循環修飾組蛋白H3多重檢測套組 (組) 廠牌:Epigentek 96 rxn

EpiQuik Circulating Modified Histone H3 Multiplex Assay

  • 同時偵測 22 種 H3 Histone 修飾,包含重要的及已知確認的修飾種類
  • 快速有效的實驗方式,只需 2 小時 30 分即可完成檢測
  • 創新的呈色方式,不須放射標定、電泳分離、層析或者昂貴的分析設備
  • 高靈敏度,每個 well 只需要 0.5 ng 的修飾目標即可偵測到
  • 附有Total histone H3 sets,可進行樣品間的 normalizing
  • 內含 Standard Control作為實驗對照

說明書下載

P-3106-96 EpiQuik Circulating Modified Histone H3 Multiplex Assay Kit 使用手冊
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