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Chromatin Preparation & Cleanup

染色質萃取試劑


染色質製備及免疫沉澱實驗流程

  1. 用 ChromaFlash (Plant) Chromatin Extraction Kit 可獲得 chromatin 產物。
  2. 用 ChromaFlash Chromatin Shearing Kit 或是 EpiSonic 2000 Sonication System 將 chromatin 片段化。
  3. 以 ChromaFlash High Sensitivity ChIP Kit 進行免疫沈澱。
  1. Upadhyaya B et. al. (April 2017). Prenatal Exposure to a Maternal High-Fat Diet Affects Histone Modification of Cardiometabolic Genes in Newborn Rats. Nutrients. 9(4)

  2. Gonzalez-Vasconcellos I et. al. (February 2017). The Rb1 tumour suppressor gene modifies telomeric chromatin architecture by regulating TERRA expression. Sci Rep. 7:42056.

  3. Bhargavan B et. al. (November 2015). Toll-like receptor-3 mediates HIV-1 transactivation via NFκB and JNK pathways and histone acetylation, but prolonged activation suppresses Tat and HIV-1 replication. Cell Signal.

  4. Liu L et. al. (March 2015). Knockdown of SALL4 enhances all-trans retinoic acid-induced cellular differentiation in acute myeloid leukemia cells. J Biol Chem.

  5. Gonzalez-Vasconcellos I et. al. (January 2015). Telomere Chromatin Condensation Assay (TCCA): a novel approach to study structural telomere integrity. Mutat Res. 771:51-5.

  6. Weyler L et. al. (December 2014). Restriction Endonucleases from Invasive Neisseria gonorrhoeae Cause Double-Strand Breaks and Distort Mitosis in Epithelial Cells during Infection. PLoS One. 9(12):e114208.

  7. Ranjan R et. al. (June 2014). The Transcription Factor Nuclear Factor of Activated T Cells c3 Modulates the Function of Macrophages in Sepsis. J Innate Immun.

  8. Liu L et. al. (November 2013). Histone lysine-specific demethylase 1 (LSD1) protein is involved in Sal-like protein 4 (SALL4)-mediated transcriptional repression in hematopoietic stem cells. J Biol Chem. 288(48):34719-28.

  9. Kim KI et. al. (May 2013). Changes in the epigenetic status of the SOX-9 promoter in human osteoarthritic cartilage. J Bone Miner Res. 28(5):1050-60.

產品列表

貨號 產品 數量
P-2001-100 ChromaFlash Chromatin Extraction Kit 染色質萃取套組(組) 廠牌:EpiGentek 100 rxns
P-2022-050 ChromaFlash Plant Chromatin Extraction Kit 植物染色質萃取套組(組) 廠牌:EpiGentek 50 rxns
P-2023-100 ChromaFlash Chromatin Isolation & Shearing Kit 染色質分離和剪切套組(組) 廠牌:EpiGentek 50 rxns
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