Clinical Research Exome

實驗數據

From the leader in target enrichment, SureSelect Clinical Research Exome, developed in collaboration with researchers from Emory University and The Children's Hospital of Philadelphia, enables comprehensive coverage of the exome, 80% of Human All Exon V5 targets at 20x, and even deeper coverage of disease-associated targets as defined by databases such as the Online Mendelian Inheritance in Man (OMIM), Human Genome Mutation Database (HGMD), and NCBI's ClinVar, 10% more of targeted bases covered at 20x with only 4Gb of sequencing (Figure 1), and even more with increased sequencing.

Confidence where it matters

The SureSelect Clinical Research Exome utilizes the high-performing Human All Exon V5 as its core design with boosted coverage in disease-associated regions, enabling deep coverage of more targets compared to competitor's design when sequenced at equivalent average coverage (Figure 2A), facilitating variant calling within these targets while maintaining the balanced and comprehensive coverage of the exome (Figure 2B). This design consists of targets included in databases such as the Online Mendelian Inheritance in Man (OMIM), the Human Genome Mutation Database (HGMD) and NCBI's ClinVar. Ancestry- and identity- informative marker panels that enable better sample tracking and provide increased confidence in data reporting have also been included. In addition, the Clinical Research Exome enables customization through the addition of up to 6Mb of content using SureDesign, a simple and easy-to-use online tool for custom library design.

Superior performance from a highly efficient workflow

The SureSelect Clinical Research Exome is a highly optimized design that provides comprehensive analysis of protein-coding regions, enabling confident variant calling with the least amount of sequencing, only 4Gb for exomes. When paired with highly stringent SureSelect workflows that enable greatly reduced hybridization times, as little as 90 minutes, this exome provides 2.5x greater throughput (Figure 3) compared to competing products with a similar capture size. Data generated can easily be analyzed using SureCall, Agilent's analysis software, that generates variant calls from raw data in hours enabling greatly reduced time to results.

產品列表

SureSelect Clinical Research Exome

Expert-optimized content
• Contains additional targets identified in collaboration with researchers from Emory University and The Children’s Hospital of Philadelphia
Most comprehensive design
• Gain deep and comprehensive coverage with only 4 Gb: 80% at 20x, 10% more in disease-associated regions
Fast track to answers
• Shorten your day with 2.5x faster workflow and break the analysis bottleneck with SureCall software