HaloPlexHS


The detection of somatic variants in heterogeneous cell populations is crucial in a wide variety of applications such as cancer genetics and immunological diversity. Unlike inherited mutations, somatic variants often occur at low allele frequencies that require sensitive methods for detection. HaloPlexHS is a high sensitivity amplicon-based targeted sequencing method based on the HaloPlex technology that incorporates molecular barcodes in the DNA library, allowing for the identification of duplicate reads hence significantly improving base calling accuracy even at low allelic frequencies compared to conventional NGS methods. Combined with the flexibility of panel customization up to 5Mb and an accelerated workflow of less than 6hr starting with 50ng gDNA, HaloPlexHS is well-suited for accurate rare variant detection in clinical research.

介紹

實驗流程


HaloPlexHS is a high sensitivity targeted sequencing method based on the HaloPlex next generation PCR technology that incorporates >106 unique molecular barcodes in the DNA library, allowing for the identification of duplicate reads hence significantly improving base calling accuracy even at low allelic frequencies compared to conventional NGS methods. Figure 1 shows the detection of allele frequencies down to 0.5% in HapMap mixtures at fixed ratios.


Excellent Performance and Accuracy

HaloPlexHS provides high coverage of target bases and on-target specificity plus excellent uniformity in both high quality and FFPE samples, ensuring that variants of interest are detected without having to perform excessive sequencing (Figures 2A and B). In addition to the unique molecular barcoding of each DNA library fragment, superior accuracy is achieved by the targeting of both DNA strands which allows for differentiation of true variants from PCR or formalin-fixation artifacts. Combined with the ability to easily create gene panels of interest through customization using SureDesign and compatibility with leading benchtop sequencing platforms, HaloPlexHS is a flexible and high sensitivity assay well-suited for clinical research.


Accelerated Solution

SureCall 3.0 software is optimized for the analysis of NGS data generated using HaloPlexHS. SureCall performs alignment, de-duplication of reads and variant calling using streamlined, guided workflows (Figure 3). When taken together with an efficient HaloPlexHS workflow which allows for complete target enrichment in less than 6 hours, time to results is reduced.

產品列表

貨號 產品 數量
G9931C HaloPlexHS 1-500kb, ILMFST 48 rxns
G9931B HaloPlexHS 1-500kb, ILMFST 96 rxns
G9941C HaloPlexHS 501kb - 2.5Mb, ILM 48 rxns
G9941B HaloPlexHS 501kb - 2.5Mb, ILM 96 rxns
G9951C HaloPlexHS 2.6Mb - 5Mb, ILM 48 rxns
G9951B HaloPlexHS 2.6Mb - 5Mb, ILM 96 rxns
G9932C HaloPlexHS 1-250kb, ION 48 rxns
G9932B HaloPlexHS 1-250kb, ION 96 rxns
G9942C HaloPlexHS 251kb - 2.5Mb, ION 48 rxns
G9942B HaloPlexHS 251kb - 2.5Mb, ION 96 rxns
5190-8629 HaloPlexHS Prepack Reagents, ILMN 16 rxns
5190-8630 HaloPlexHS Prepack Reagents, ILMN 48 rxns
5190-8631 HaloPlexHS Prepack Reagents, ILMN 96 rxns
5190-8632 HaloPlexHS Prepack Reagents, ION 16 rxns
5190-8633 HaloPlexHS Prepack Reagents, ION 48 rxns
5190-8634 HaloPlexHS Prepack Reagents, ION 96 rxns

HaloPlexHS

    Unparalleled Sensitivity
  • Uniquely tag DNA fragments with more than a million 10-nt molecular barcodes
  • Confidently detect mutations present at below 1% allele frequency in genetically heterogeneous samples
  • Superior Accuracy
  • Differentiation of true variants from PCR or formalin fixation artifacts by targeting both DNA strands
  • High on-target specificity and deep coverage of target bases so key variants are not missed
  • Accelerated Solution
  • Complete target enrichment in less than 6hr from only 50ng of gDNA
  • From raw data to categorized mutations in 3 steps using SureCall data analysis software
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