The detection of somatic variants in heterogeneous cell populations is crucial in a wide variety of applications such as cancer genetics and immunological diversity. Unlike inherited mutations, somatic variants often occur at low allele frequencies that require sensitive methods for detection. HaloPlexHS is a high sensitivity amplicon-based targeted sequencing method based on the HaloPlex technology that incorporates molecular barcodes in the DNA library, allowing for the identification of duplicate reads hence significantly improving base calling accuracy even at low allelic frequencies compared to conventional NGS methods. Combined with the flexibility of panel customization up to 5Mb and an accelerated workflow of less than 6hr starting with 50ng gDNA, HaloPlexHS is well-suited for accurate rare variant detection in clinical research.
HaloPlexHS is a high sensitivity targeted sequencing method based on the HaloPlex next generation PCR technology that incorporates >106 unique molecular barcodes in the DNA library, allowing for the identification of duplicate reads hence significantly improving base calling accuracy even at low allelic frequencies compared to conventional NGS methods. Figure 1 shows the detection of allele frequencies down to 0.5% in HapMap mixtures at fixed ratios.
HaloPlexHS provides high coverage of target bases and on-target specificity plus excellent uniformity in both high quality and FFPE samples, ensuring that variants of interest are detected without having to perform excessive sequencing (Figures 2A and B). In addition to the unique molecular barcoding of each DNA library fragment, superior accuracy is achieved by the targeting of both DNA strands which allows for differentiation of true variants from PCR or formalin-fixation artifacts. Combined with the ability to easily create gene panels of interest through customization using SureDesign and compatibility with leading benchtop sequencing platforms, HaloPlexHS is a flexible and high sensitivity assay well-suited for clinical research.
SureCall 3.0 software is optimized for the analysis of NGS data generated using HaloPlexHS. SureCall performs alignment, de-duplication of reads and variant calling using streamlined, guided workflows (Figure 3). When taken together with an efficient HaloPlexHS workflow which allows for complete target enrichment in less than 6 hours, time to results is reduced.
貨號 | 產品 | 數量 | |
---|---|---|---|
G9931C | HaloPlexHS 1-500kb, ILMFST | 48 rxns | |
G9931B | HaloPlexHS 1-500kb, ILMFST | 96 rxns | |
G9941C | HaloPlexHS 501kb - 2.5Mb, ILM | 48 rxns | |
G9941B | HaloPlexHS 501kb - 2.5Mb, ILM | 96 rxns | |
G9951C | HaloPlexHS 2.6Mb - 5Mb, ILM | 48 rxns | |
G9951B | HaloPlexHS 2.6Mb - 5Mb, ILM | 96 rxns | |
G9932C | HaloPlexHS 1-250kb, ION | 48 rxns | |
G9932B | HaloPlexHS 1-250kb, ION | 96 rxns | |
G9942C | HaloPlexHS 251kb - 2.5Mb, ION | 48 rxns | |
G9942B | HaloPlexHS 251kb - 2.5Mb, ION | 96 rxns | |
5190-8629 | HaloPlexHS Prepack Reagents, ILMN | 16 rxns | |
5190-8630 | HaloPlexHS Prepack Reagents, ILMN | 48 rxns | |
5190-8631 | HaloPlexHS Prepack Reagents, ILMN | 96 rxns | |
5190-8632 | HaloPlexHS Prepack Reagents, ION | 16 rxns | |
5190-8633 | HaloPlexHS Prepack Reagents, ION | 48 rxns | |
5190-8634 | HaloPlexHS Prepack Reagents, ION | 96 rxns |