HaloPlex Custom Kits
HaloPlex custom kits provide you with unparalleled flexibility to create panels focused on your genomic regions of interest for any application. Using a simple, library prep-free workflow, complete target enrichment is achieved in less than 6 hours from just 200ng of input DNA. HaloPlex offers superior specificity and coverage that allows you to confidently identify genetic variants, faster.
介紹
HaloPlex™ Target Enrichment System enables fast, simple and efficient analysis of genomic regions of interest in a large number of samples. Custom designs, for panels containing thousand of exons, are easily created with SureDesign. By combining single-tube target amplification and removing the need for library preparation, the total sample preparation time and cost is reduced without the need for dedicated instrumentation or automation. The availability of 96 indexes and compatibility with different desktop sequencing and high-throughput platforms provide you the flexibility you need to run your experiments. Finally, the assay has premium performance, with high specificity, uniformity and variant detection.
HaloPlex - Performance
HaloPlex technology combines the robustness of hybridization, which allows flexibility in number of target regions, with the high specificity and speed of PCR. It provides premium performance, with great specificity, uniformity and high sensitivity for variant calling.
Premium performance obtained for several design sizes
Great coverage for contiguous or exonic regions
HaloPlex delivers high specificity, uniformity, and sequencing coverage for any design size, and across all target regions. (A) Enrichment of all exons within the CFTR gene, for 5 different samples. (B) Contiguous and highly repetitive region (~160kb) enriched with HaloPlex.產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| 5190-7322 | HaloPlex Enrichment Control DNA | 16 rxns | |
| 5190-8065 | HaloPlex Prepack Reagents ILM | 16 rxns | |
| 5190-7325 | HaloPlex Prepack Reagents ION | 16 rxns | |
| G9901C | HaloPlex 1-500kb, ILMFST | 48 rxns | |
| G9901B | HaloPlex 1-500kb, ILMFST | 96 rxns | |
| G9911C | HaloPlex 501kb-2.5Mb, ILM | 48 rxns | |
| G9911B | HaloPlex 501kb-2.5Mb, ILM | 96 rxns | |
| G9921C | HaloPlex 2.6Mb-5Mb, ILM | 48 rxns | |
| G9921B | HaloPlex 2.6Mb-5Mb, ILM | 96 rxns | |
| G9902C | HaloPlex 1-250kb, ION | 48 rxns | |
| G9902B | HaloPlex 1-250kb, ION | 96 rxns | |
| G9912C | HaloPlex 251kb-2.5Mb, ION | 48 rxns | |
| G9912B | HaloPlex 251kb-2.5Mb, ION | 96 rxns |
HaloPlex Custom Kits
- Available in 48 or 96 reaction sizes
- Create designs up to 5Mb in size (up to 200,000 aplicons)
- Contains all reagents needed to perform sample preparation - including primers, probes, buffers and indexes/barcodes to multiplex up to 96 samples
- Process 96 samples in a day with Agilent Automation Solutions
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