SureSelectQXT Reagent Kits
The SureSelect Target Enrichment System is the most proven target enrichment solution today. This technology enables the capture of genomic targets using long 120nt RNA baits which allow for efficient enrichment of regions of interest facilitating confident variant calling.
The SureSelectQXT Reagent Kit is a combined shearing-free transposase-based library prep and target enrichment solution that enables a 90-minute hybridization step, the fastest in the market, resulting in sequencing-ready libraries in less than a day. This highly streamlined protocol which requires only 3.5hrs of hands-on time. With one library per capture, the generation of high quality sequencing-ready libraries from only 50ng sample input is enabled providing faster time-to-answers.
內容描述
Assays of high sensitivity and minimal turn-around time that generate unambiguous results from minimal sample input are becoming essential. From the leader in target enrichment, SureSelectQXT reagent kits enable single molecule resolution of variants with a streamlined same day sample to sequencing workflow from only 50 ng sample input. This is at least 3.5x faster with 30% less hands-on time compared to other transposase-based enrichment products (Figure 1).
Exceptional coverage and uniformity from SureSelectQXT
Figure 1. Excellent coverage of targeted bases at 20x coupled with superior on-target metric (A) and exceptional uniformity (B) enables decreased sequencing requirement while enabling confident variant calling (SureSelect Human All Exon V5, 4 Gb sequencing)Streamlined workflow
SureSelectQXT kits for Illumina sequencing couple a convenient, shearing-free, transposase-based library prep protocol with a quick 90 minute hybridization technology for the only same day sample to sequencing capture-based enrichment solution in the market (Figure 2). This revolutionary workflow greatly accelerates turn-around time from sample to data while providing deep coverage of genomic regions of interest, greatly advancing clinical research sequencing. SureSelectQXT requires only 50 ng of sample input, allowing analysis of a wide range of sample types including those with limited availability. These features allow generation of data with high sensitivity and accuracy for confident variant calling of every sample, every single time.
Uncompromised performance
Exome enrichment using SureSelectQXT kits provides coverage of 80% of targeted bases at 20x, providing support for variant calling, from only 4 Gb of sequencing. SureSelectQXT’s superior performance in variant detection is enabled by SureSelect’s proven hybridization technology, optimized to reduce hybridization time down to only 90 minutes, and the comprehensive exome design. Together, these ensure complete and accurate profiling of genomic regions of interest with the least amount of sequencing (figure 3).
Accelerated discovery for your regions of interest
Easily create custom panels using SureDesign, a web-based tool that supports creation of custom target enrichment panels allowing focused interrogation of regions of interest. Faster time to results for profiling exomes or custom genomic targets is enabled by SureCall, a simplified raw data to variant analysis software.
產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| G9681A | SureSelectQXT Reagent Kit | 16 rxns | |
| G9681B | SureSelectQXT Reagent Kit | 96 rxns | |
| G9683A | SureSelectQXT Reagent Kit, NSQ | 16 rxns | |
| G9683B | SureSelectQXT Reagent Kit, NSQ | 96 rxns |
SureSelectQXT Reagent Kits
- Enables enrichment of exomes, gene panels or custom targets (up to 24Mb) with a 90-minute hyb generating sequencing-ready libraries in less than a day.
- Easy transposase-based library prep workflow compatible with 50ng input.
- Reagent kits provide reagents for library prep as well as target enrichment including dual index barcodes, baits ordered separately.
- Available in 16 and 96rxn kits. The 96 reaction kits are compatible with manual or automated processing.
- Library prep only kits enable whole-genome sequencing for human or non-human samples, supporting protocols provided
- Compatible with standard 3ug input for highest complexity or lower 200ng input for samples of low availability. Automation-compatible protocols are also provided
- Customization is enabled by SureDesign and faster time to results by SureCall, a simplified raw data to variant analysis software.
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