Methyl-Seq
Agilent SureSelectXT Human Methyl-Seq is the first comprehensive target enrichment system to enable researchers to focus on the regions where methylation is known to impact gene regulation. It targets the most complete content for methylation sequencing, including cancer tissue-specific DMRs, GENCODE promoters, CpG islands, shores and shelves ±4kb, DNaseI hypersensitive sites and RefGenes. Focus on key epigenetic regions with target enriched bisulfite sequencing for faster analysis and higher coverage.
Design: 84Mb design covering 3.7 million CpGs
- CpG islands
- Cancer, tissue specific DMRs
- Gencode promoters
- DMRs or regulatory feature in:
- CpG islands, shores, and shelves ±4kb
- DNase I hypersensitive sites
- Refseq genes
- Ensembl regulatory features
Workflow: Optimized DNA methylation analysis using the SureSelect Target Enrichment System
Performance: High-sensitivity with single-base resolution
產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| G9651A | SureSelectXT Methyl-Seq Reagent kit, HSQ | 16 rxns | |
| G9651B | SureSelectXT Methyl-Seq Reagent kit, HSQ | 96 rxns | |
| G9651C | SureSelectXT Methyl-Seq Reagent kit, HSQ | 480 rxns | |
| 5190-4661 | SureSelectXT Human Methyl-Seq | 16 rxns | |
| 5190-4662 | SureSelectXT Human Methyl-Seq | 96 rxns | |
| 5190-4663 | SureSelectXT Human Methyl-Seq | 480 rxns |
Methyl-Seq
- Delivers more information than methylation microarrays by detecting individual CpGs
- Increases throughput, reduces costs compared to whole-genome bisulfite sequencing (WGBS)
- Reveals methylated regions undetected by reduced representation bisulfite sequencing (RRBS) and Methylated DNA Immunoprecipitation (MeDIP)
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