SureSelect Strand Specific RNA Library
安捷倫的 SureSelect Strand Specific RNA Library Preparation Kit 是製備 mRNA 或 targeted RNA-seq Library 的高度敏感性及專一性方法，可以更有效地了解基因的調控情形。
引用文獻
- YAP is essential for mechanical force production and epithelial cell proliferation during lung branching morphogenesis Chuwen Lin, Erica Yao, Kuan Zhang, Xuan Jiang, Stacey Croll, Katherine Thompson-Peer, Pao-Tien Chuang eLife. 2017; 6: e21130. Published online 2017 Mar 21.
- Effect of age on pro-inflammatory miRNAs contained in mesenchymal stem cell-derived extracellular vesicles J. Fafián-Labora, I. Lesende-Rodriguez, P. Fernández-Pernas, S. Sangiao-Alvarellos, L. Monserrat, O. J. Arntz, F. J. Van de Loo, J. Mateos, M. C. Arufe Sci Rep. 2017; 7: 43923. Published online 2017 Mar 6.
- Endometrial factors similarly induced by IFNT2 and IFNTc1 through transcription factor FOXS1 Kazuya Kusama, Rulan Bai, Keigo Nakamura, Sayaka Okada, Jiro Yasuda, Kazuhiko Imakawa PLoS One. 2017; 12(2): e0171858. Published online 2017 Feb 15.
- Prediction and prioritization of neoantigens: integration of RNA sequencing data with whole‐exome sequencing Takahiro Karasaki, Kazuhiro Nagayama, Hideki Kuwano, Jun‐ichi Nitadori, Masaaki Sato, Masaki Anraku, Akihiro Hosoi, Hirokazu Matsushita, Masaki Takazawa, Osamu Ohara, Jun Nakajima, Kazuhiro Kakimi Cancer Sci. 2017 Feb; 108(2): 170–177.
- Transcriptional Analysis of Acinetobacter sp. neg1 Capable of Degrading Ochratoxin A Vania C. Liuzzi, Francesca Fanelli, Mariana Tristezza, Miriam Haidukowski, Ernesto Picardi, Caterina Manzari, Claudia Lionetti, Francesco Grieco, Antonio F. Logrieco, Michael R. Thon, Graziano Pesole, Giuseppina Mulè Front Microbiol. 2016; 7: 2162.
- Transcriptional signatures of somatic neoblasts and germline cells in Macrostomum lignano Magda Grudniewska, Stijn Mouton, Daniil Simanov, Frank Beltman, Margriet Grelling, Katrien de Mulder, Wibowo Arindrarto, Philipp M. Weissert, Stefan van der Elst, Eugene Berezikov eLife. 2016; 5: e20607.
- Transcriptomic Changes in Liver of Young Bulls Caused by Diets Low in Mineral and Protein Contents and Supplemented with n-3 Fatty Acids and Conjugated Linoleic Acid Sara Pegolo, Alessio Cecchinato, Núria Mach, Massimiliano Babbucci, Marianna Pauletto, Luca Bargelloni, Stefano Schiavon, Giovanni Bittante PLoS One. 2016; 11(12): e0167747.
- FOXA1, GATA3 and PPARɣ Cooperate to Drive Luminal Subtype in Bladder Cancer: A Molecular Analysis of Established Human Cell Lines Joshua I. Warrick, Vonn Walter, Hironobu Yamashita, Eunah Chung, Lauren Shuman, Vasty Osei Amponsa, Zongyu Zheng, Wilson Chan, Tiffany L. Whitcomb, Feng Yue, Tejaswi Iyyanki, Yuka I. Kawasawa, Matthew Kaag, Wansong Guo, Jay D. Raman, Joo-Seop Park, David J. DeGraff Sci Rep. 2016; 6: 38531.
- Loss of EGFR-ASAP1 signaling in metastatic and unresectable hepatoblastoma Sarangarajan Ranganathan, Mylarappa Ningappa, Chethan Ashokkumar, Brandon W. Higgs, Jun Min, Qing Sun, Lori Schmitt, Shankar Subramaniam, Hakon Hakonarson, Rakesh Sindhi Sci Rep. 2016; 6: 38347.
- Identification of novel targets of diabetic nephropathy and PEDF peptide treatment using RNA-seq Ana Rubin, Anna C. Salzberg, Yuka Imamura, Anzor Grivitishvilli, Joyce Tombran-Tink BMC Genomics. 2016; 17: 936.
內容描述
RNA library prep kit 提供相當高的序列針對性 ( strand specificity )，在整個轉錄產物上具有更高的覆蓋均勻性，進而能更好的探討基因調控現象。高度的序列針對性更能發現新的轉錄產物和基因融合體。此試劑套組即使用於少量樣品的情況下 ( total RNA 濃度低至 50ng )，依舊可維持高度的 library 複雜性，對於樣品有限的研究是相當重要的。
RNA-Seq 和基因調控研究的完整套組
NGS 技術現正被廣泛應用於基因調控的研究，strand specific 訊息更能進一步提供完整訊息。SureSelect Strand Specific RNA Library Prep Kit 是一套完整的試劑組，包括預混合試劑 ( master-mixed reagents ) 和簡化的工作流程，能減少實驗操作時間並能更快的獲得結果。SureSelect RNA Library Preparation Kit 是安捷倫 NGS 基因調控完整分析方案的重要部分，其包括 SureSelect RNA Target Enrichment, SureSelect Human Methyl-Seq 和 GeneSpring NGS data analysis 等。
產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| G9691A | SureSelect Strand Specific RNA Library | 16 rxns | |
| G9691B | SureSelect Strand Specific RNA Library | 96 rxns | |
| G9692A | SureSelectXT RNA Reagent Kit, ILM | 16 rxns | |
| G9692B | SureSelectXT RNA Reagent Kit, ILM | 96 rxns |
SureSelect Strand Specific RNA Library
- 高度 strand specificity 可辨別重疊的轉錄產物並研究 antisense 轉錄情形。
- 少量的起始樣品 ( total RNA 濃度低至 50ng ) 仍可得到良好的 library 複雜性。
- 最佳的覆蓋均勻性減少 5’/3’ 偏差。
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