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SureSelectXT HS Target Enrichment
For High Sensitivity SureSelect Library Prep

超微量目標外顯子組富集系統


SureSelectXT HS 是安捷倫 SureSelect 外顯子捕獲系統中製備 library 的最新產品,擁有最高靈敏度的 NGS library 製備及 target enrichment 效力。

各種 SureSelectXT 系列產品比較表
SureSelect Library Prep Solutions for Cancer and Constitutional Applications
Product NameSureSelectXT HSSureSelectXT Low InputSureSelectXTSureSelectXT2SureSelectQXT
DNA Input10 ng - 200 ng10 ng - 200 ng200 ng - 3 µg100 ng - 1 µg50 ng
Turnaround Time8 hours8 hours1.5 day1.5 day7 hours
Covaris NeededYesYesYesYesNo
Library ComplexityHighestHighestHighMediumMedium
Unique FeaturesFFPE optimized
Molecular barcodes
Mastermixed reagents
Samples indexed prior to capture eliminating concern of cross sample contamination
FFPE optimized
Molecular barcodes (optional)
Mastermixed reagents
Samples indexed prior to capture eliminating concern of cross sample contamination
Compatible with FFPE samples
Robust variant identification
Pre-capture pooling
Mastermix reagents
Transposase-based
Mastermix reagents
Whole genome sequencing and target enrichment compatible
For intact DNA only
Key BenefitsHigh sensitivity for ≤1% VAF192 sample indexesHigh-complexity libraries for rare allele detectionCost-effectiveCovaris-free workflow

FFPE-optimized library prep

從 NGS 的樣品來源而言,通常 FFPE DNA 用於製備 library 時,其 DNA 的降解多太嚴重。因此,重要的是優化 library 製備流程並最大限度地減少樣品流失的步驟,以從樣品中製作高品質的 library。您的 FFPE 樣品透過 SureSelectXT HS 皆能產生複雜的 library,且起始材料只需 10 ~ 200 ng 即可。

SureSelectXT HS optimized performance for FFPE with the majority of bases covered at 20X across decreasing sample quality. % bases covered by at least 20X read depth with increasing sequencing depth (4-8 million - 2x100 bp reads) is plotted against gDNA quality (qPCR-based gDNA quality (ddCq) and is calculated using Agilent’s FFPE QC Assay kit).

Superior sensitivity

在大多數情形下,偵測靈敏度受限於起始材料含量、分析效能、PCR 和測序錯誤率。SureSelectXT HS 結合分子標記 ( Molecular barcode ),能過濾掉在 library 製備、target enrichment 和定序過程中產生的人工誤差 ( artifacts ) 而導致的偽陽性變異 ( false positive variants )。SureSelectXT HS 的分子標記為業界領先的指標,可讓檢測變異頻率的解析度降至 ≤ 1%。

Detect down to 1% allele frequency. SureSelectXT HS generated library sequenced to 3000X depth produces high correlation of observed to expected frequency down to as little as 1% VAF (HD200 Quantitative Multiplex Reference Standard and ClearSeq Comprehensive Cancer Panel).

Expedite analysis with a streamlined workflow

SureSelectXT HS減少酵素催化、清洗和樣品轉移的步驟,可簡化實驗流程並維持 library 的高複雜性。此套組的雜合反應時間為市場最快,90分鐘雜合時間結合預混合試劑 ( master-mixed reagents ),能在一天內從樣品中完成 sequencing-ready library 的製備。另外,一次的工作流程可同時支援多達32個樣品的深度多重測序。

產品列表

貨號 產品 數量
G9702A SSEL XT HS Reagent Kit (1-16) 16 rxn
G9702B SSEL XT HS Reagent Kit (17-32) 16 rxn
G9702C SSEL XT HS Reagent Kit (1-32) 96 rxn
G9703A SSEL XT Low Input Reagent Kit (1-96)
G9703B SSEL XT Low Input Reagent Kit (97-192)

SureSelectXT HS Target Enrichment

  • 只需 10ng DNA 即可進行實驗。
  • 適用於完整 DNA、低品質或高品質的 FFPE DNA。
  • 分子標記 ( Molecular barcode, MBC ) 有效提升 library 的陽性預測值 ( positive predictive value, PPV )。
  • 高複雜度的 library 在目標區域有更高比例的 reads 數。
  • 雜合時間只需 90 分鐘,同時採用預混合試劑 ( master-mixed reagents ) 使實驗流程更快更有效率。

說明書下載

SureSelectXT HS Target Enrichment 原廠英文 Brochure
SureSelectXT HS Target Enrichment 中文產品型錄
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