SureSelectXT HS 是安捷倫 SureSelect 外顯子捕獲系統中製備 library 的最新產品,擁有最高靈敏度的 NGS library 製備及 target enrichment 效力。
SureSelect Library Prep Solutions for Cancer and Constitutional Applications | |||||
Product Name | SureSelectXT HS | SureSelectXT Low Input | SureSelectXT | SureSelectXT2 | SureSelectQXT |
DNA Input | 10 ng - 200 ng | 10 ng - 200 ng | 200 ng - 3 µg | 100 ng - 1 µg | 50 ng |
Turnaround Time | 8 hours | 8 hours | 1.5 day | 1.5 day | 7 hours |
Covaris Needed | Yes | Yes | Yes | Yes | No |
Library Complexity | Highest | Highest | High | Medium | Medium |
Unique Features | FFPE optimized Molecular barcodes Mastermixed reagents Samples indexed prior to capture eliminating concern of cross sample contamination | FFPE optimized Molecular barcodes (optional) Mastermixed reagents Samples indexed prior to capture eliminating concern of cross sample contamination | Compatible with FFPE samples Robust variant identification | Pre-capture pooling Mastermix reagents | Transposase-based Mastermix reagents Whole genome sequencing and target enrichment compatible For intact DNA only |
Key Benefits | High sensitivity for ≤1% VAF | 192 sample indexes | High-complexity libraries for rare allele detection | Cost-effective | Covaris-free workflow |
從 NGS 的樣品來源而言,通常 FFPE DNA 用於製備 library 時,其 DNA 的降解多太嚴重。因此,重要的是優化 library 製備流程並最大限度地減少樣品流失的步驟,以從樣品中製作高品質的 library。您的 FFPE 樣品透過 SureSelectXT HS 皆能產生複雜的 library,且起始材料只需 10 ~ 200 ng 即可。
SureSelectXT HS optimized performance for FFPE with the majority of bases covered at 20X across decreasing sample quality. % bases covered by at least 20X read depth with increasing sequencing depth (4-8 million - 2x100 bp reads) is plotted against gDNA quality (qPCR-based gDNA quality (ddCq) and is calculated using Agilent’s FFPE QC Assay kit).在大多數情形下,偵測靈敏度受限於起始材料含量、分析效能、PCR 和測序錯誤率。SureSelectXT HS 結合分子標記 ( Molecular barcode ),能過濾掉在 library 製備、target enrichment 和定序過程中產生的人工誤差 ( artifacts ) 而導致的偽陽性變異 ( false positive variants )。SureSelectXT HS 的分子標記為業界領先的指標,可讓檢測變異頻率的解析度降至 ≤ 1%。
Detect down to 1% allele frequency. SureSelectXT HS generated library sequenced to 3000X depth produces high correlation of observed to expected frequency down to as little as 1% VAF (HD200 Quantitative Multiplex Reference Standard and ClearSeq Comprehensive Cancer Panel).SureSelectXT HS減少酵素催化、清洗和樣品轉移的步驟,可簡化實驗流程並維持 library 的高複雜性。此套組的雜合反應時間為市場最快,90分鐘雜合時間結合預混合試劑 ( master-mixed reagents ),能在一天內從樣品中完成 sequencing-ready library 的製備。另外,一次的工作流程可同時支援多達32個樣品的深度多重測序。
貨號 | 產品 | 數量 | |
---|---|---|---|
G9702A | SSEL XT HS Reagent Kit (1-16) | 16 rxn | |
G9702B | SSEL XT HS Reagent Kit (17-32) | 16 rxn | |
G9702C | SSEL XT HS Reagent Kit (1-32) | 96 rxn | |
G9703A | SSEL XT Low Input Reagent Kit (1-96) | ||
G9703B | SSEL XT Low Input Reagent Kit (97-192) |