ChromaFlash High-Sensitivity ChIP Kit
高敏感性染色質免疫沉澱
只需少量的哺乳動物細胞或者組織即可進行染色質免疫沉澱實驗
ChromaFlash™ High-Sensitivity ChIP Kit 提供完整的試劑組合可讓您從動物細胞或者組織來進行高通量的染色質免疫沉澱實驗，高專一性與高敏感性的優勢讓您從染色質汲取特定DNA片段，最適化調整的流程與配方可以有效降低非專一雜質。使用 ChromaFlash™ High-Sensitivity ChIP Kit 獲得的蛋白結合 DNA 能進行各種下游實驗，例如 PCR (ChIP-PCR)、microarray (ChIP-on-chip) 及次世代定序 (ChIP-seq) 等實驗。
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ChIP kit 包含 positive control 抗體 (RNA polymerase II)、negative control non-immune IgG 和 GAPDH primers 可用於進行試劑套組的 positive control 效能與步驟驗證，在大多成長的動物細胞中，GAPDH 基因啟動子會聚集RNA polymerase II，所以能透過 RNA polymerase II 的抗體來抓取，反之 non-immune IgG 則不會被抓取，免疫沉澱後可將和蛋白結合的 DNA 沖洗下來，並且進行許多後續的實驗分析。
實驗流程
Low abundance protein enrichment: Sheared chromatin isolated from different numbers of MCF-7 cells was used for ChIP-qPCR analysis of ER-a enrichment in TFF1 promoters using the ChromaFlash High-Sensitivity ChIP Kit (Cat, No. P-2027) and the EpiQuik Quantitative PCR Fast Kit (Cat. No. P-1029).
High abundance protein enrichment: Sheared chromatin isolated from different numbers of MBD-231 cells was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters using the ChromaFlash High-Sensitivity ChIP Kit (Cat. No. P-2027) and the EpiQuik Quantitative PCR Fast Kit (Cat. No. P-1029).
產品列表
| 貨號 | 產品 | 數量 | |
|---|---|---|---|
| P-2027-24 | ChromaFlash High-Sensitivity ChIP Kit 高靈敏性免疫沉澱試劑套組(組) 廠牌:Epigentek | ||
| P-2027-48 | ChromaFlash High-Sensitivity ChIP Kit 高靈敏性免疫沉澱試劑套組(組) 廠牌:Epigentek |
ChromaFlash High-Sensitivity ChIP Kit
- 高度專一性目標汲取效果 positive 和 negative control 的比值 > 500，非常少量的細胞 (~2000 個細胞) 即可進行 ChIP 實驗。
- 最適化的試劑配方與流程有效降低 ChIP 的背景非專一雜訊。
- 獨特的 chimeric proteins 覆蓋於Strip-wells使之有最高量的 IgG binding domains，能讓抗體的專一選擇性與抓取力達到最高效能。
- 從 chromatin 到最後的 DNA 洗出流程只需花費 5 小時。
- 96-well plate 的規格讓實驗更具彈性，可一次進行一個反應或者一次進行24-48個反應。
- 可對應各種下游實驗，包含ChIP-PCR、ChIP-on-chip 和 ChIP-seq等。
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